Blood Vial

After collecting cord blood, the blood is stored in bags or bottles, it is taken to the bank cord blood through the courier. Once he reached the shore, the sample is given a unique identification number. Subsequently, physicians separate the stem cells of blood and cryogenically stored (frozen in liquid nitrogen between them). After that, if appropriate, the cord blood stem cells are thawed and used in autologous or allogeneic procedures or procedures.

Cryopreservation

During storage of cord blood, each bottle is labeled with the number of cord blood, the baby's name, date of birth, date of processing, and content. Then he enters the first stage of cryopreservation. The bottles are placed in two cryo-tanks with different temperatures and are then immersed in tanks containing liquid nitrogen. This step ensures the safety of stem cells. The series of temperature prevents the collapse of stem cells to undergo a shock due to the intense negative temperature - 32 degrees Fahrenheit.

The freezing of liquid nitrogen cord blood-

To prevent ice crystals forming on the stem cells, it is important to freeze the cells gradually. To do this, including a freezer controlled rate is used. The process and freezers are designed to prevent "Transient Warming Events (TWES). If there is a fluctuation in temperature, the cells will suffer irreparable harm.

While the freezing of living tissue, they are soaked in a solvent that protects cells against the formation of ice crystals. The ice crystals can break the cell membranes. The most common solvent used for cryopreservation is DiMethylSulOxide (DMSO), usually 10 percent solution. It serves as a shield around the cells.

Before placing the cells in vats of liquid nitrogen at a temperature of -196 degrees Celsius, the cells are exposed to a gradual freezing process in a steam reservoir, containing liquid nitrogen for up to 25 inches ensure that the temperature remains above temperature. All material is placed in a very controlled and secure.

Types of Freezer

Some of the public banks of cord blood with budgets heavy use of "BioArchive" freezers. They are liquid nitrogen freezers using a patented process, a robotic system controlled by a computer for cryopreservation and store a maximum of 3626 units bag. The location and retrieval of each unit is computerized.

The goal of the robotic arm is to prevent "Transient Warming Events (TWES), where blood samples are kept or removed from the freezer. The majority of the public banks of cord blood in America is using" dewars ". They are isolated tanks with lids. The main disadvantage of these tanks is that the lid must be lifted open every time you add or remove samples. The temperatures are under constant revision and very effective for prolonged storage.

The contents of the freezer

Cord blood is stored in containers or bags. When the freezer door to get loaded with containers of cord blood, they must be equally spaced from each other to ensure the stability of the temperature. The freezer has racks to hold the two bags and bottles, but it is not recommended to mix containers in a freezer. Consequently, most banks focus on one type of container. The cold nitrogen maintains the cryogenic temperature. Some cord blood banks prefer liquid nitrogen while others prefer cold nitrogen vapor.

There are advantages, and disadvantages of the two types of nitrogen.

Advantages and disadvantages of liquid nitrogen

Use a larger amount of nitrogen
Maintains a constant temperature
There is a chance of a contagious disease documented leaking f.